運(yùn)用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時(shí)對(duì)某個(gè)信號(hào)通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測,芯片上的基因包括了與研究對(duì)象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對(duì)不同的信號(hào)通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號(hào) | 名稱 | 基因列表 | 價(jià)格 |
啟因生物(wcgene.com) | HUMAN-WC038 | Genes Interacting with CFTR qpcr array | AHSA1, CANX, CFTR, DNAJA1, DNAJC5, EZR, GOPC, HSP90AA1, HSPA4 (HSP70), HSPA8, NME1 (NM23A), PDZK1, PPP2R4, PRKAA1, PRKAA2, SLC26A3, SLC9A3R1, SLC9A3R2, SNAP23, STX1A, STX8, TGFB1, TJP1, VCP. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC037 | Genes Interacting with CFTR qpcr array | Ahsa1, Canx, Cftr, Dnaja1, Dnajc5, Ezr, Gopc, Hsp90aa1, Hspa4 (Hsp70), Hspa8, Nme1 (Nm23a), Pdzk1, Ppp2r4, Prkaa1, Prkaa2, Slc26a3, Slc9a3r1, Slc9a3r2, Snap23, Stx1a, Stx8, Tgfb1, Tjp1, Vcp. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC038 | Genes Interacting with CFTR qpcr array | Canx, Cftr, Dnaja1, Dnajc5, Ezr, Gopc, Hsp90aa1, Hspa4 (Hsp70), Nme1 (Nm23a), Pdzk1, Ppp2r4, Prkaa1, Prkaa2, Slc26a3, Slc9a3r1, Slc9a3r2, Snap23, Stx1a, Stx8, Tgfb1, Tjp1, Vcp. | 300元/樣 |
檢測流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測,檢測RNA純度及完整度,提供RNA質(zhì)量報(bào)告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實(shí)時(shí)定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計(jì)算PCR芯片中的各個(gè)Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。