運用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時對某個信號通路或疾病相關(guān)基因的多個基因的表達(dá)量變化進(jìn)行檢測,芯片上的基因包括了與研究對象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對不同的信號通路和疾病相關(guān)基因設(shè)計了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號 | 名稱 | 基因列表 | 價格 |
啟因生物(wcgene.com) | HUMAN-WC041 | Kinases & Phosphatases qpcr array | AURKA, AURKB, AURKC, CASK, CDC42BPA, CDK5, CDK5R1, CIT, LIMK1, LIMK2, MAP3K11, MAPK13,MARK2, MYLK, MYLK2, PAK1, PAK4, PPP1R12A, PPP1R12B, PPP3CA, PPP3CB, ROCK1, SSH1, SSH2. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC040 | Kinases & Phosphatases qpcr array | Aurka, Aurkb, Aurkc, Cask, Cdc42bpa, Cdk5, Cdk5r1, Cit, Limk1, Limk2, Map3k11, Mapk13,Mark2, Mylk, Mylk2, Pak1, Pak4, Ppp1r12a, Ppp1r12b, Ppp3ca, Ppp3cb, Rock1, Ssh1, Ssh2. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC041 | Kinases & Phosphatases qpcr array | Aurka, Aurkb, Aurkc, Cask, Cdc42bpa, Cdk5, Cdk5r1, Cit, Limk1, Limk2, Map3k11, Mapk13,Mark2, Mylk, Mylk2, Pak1, Pak4, Ppp1r12a, Ppp1r12b, Ppp3ca, Ppp3cb, Rock1, Ssh2. | 300元/樣 |
檢測流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測,檢測RNA純度及完整度,提供RNA質(zhì)量報告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實時定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計算PCR芯片中的各個Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。