運(yùn)用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時(shí)對(duì)某個(gè)信號(hào)通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測(cè),芯片上的基因包括了與研究對(duì)象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對(duì)不同的信號(hào)通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號(hào) | 名稱 | 基因列表 | 價(jià)格 |
啟因生物(wcgene.com) | HUMAN-WC011 | Apoptosis qpcr array | AKT1, BIRC3 (c-IAP1), CARD6, CARD9, CASP1 (ICE), CASP8 (FLICE), CD14, FADD, IFNB1, IKBKB, IL12A, IL12B, IL1B, IL6, IRAK1, JUN, MAP3K7 (TAK1), MAPK1 (ERK2), MAPK8 (JNK1), MPO, NFKB1, NFKBIA (IkBa/MAD3), PIK3CA, PYCARD, RAC1, RIPK1, RIPK2, TNF, TNFRSF1A, TRAF6. | 300元/樣 |
啟因生物(wcgene.com) | HUMAN-WC053 | Apoptosis qpcr array | ANXA5, BAX, BCL2, BCL2L1 (BCL-X), CASP1 (ICE), CASP3, CAV1, CCL2 (MCP-1), CCL5 (RANTES), CFLAR (CASPER),CX3CL1, EDN1, EDNRA, FAS (TNFRSF6), FASLG (TNFSF6), FGF2, HIF1A, HMOX1, IL1B, IL3, IL6, IL7, OCLN, PF4, PTK2, SPHK1, TEK (TIE2), THBS1, TNF, TNFSF10 (TRAIL). | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC011 | Apoptosis qpcr array | Akt1, Birc3 (c-IAP1), Card6, Card9, Casp1 (Ice), Casp8 (Flice), Cd14, Fadd, Ifnb1, Ikbkb, Il12a, Il12b, Il1b, Il6, Irak1, Jun, Map3k7 (Tak1), Mapk1 (Erk2), Mapk8 (Jnk1), Mpo, Nfkb1, Nfkbia (IkBa/Mad3), Pik3ca, Pycard, Rac1, Ripk1, Ripk2, Tnf, Tnfrsf1a, Traf6. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC052 | Apoptosis qpcr array | Anxa5, Bax, Bcl2, Bcl2l1 (Bcl-X), Casp1 (Ice), Casp3, Cav1, Ccl2 (Mcp-1), Ccl5 (Rantes), Cflar (Casper), Cradd, Cx3cl1, Edn1, Ednra, Fas (Tnfrsf6), Fasl (Tnfsf6), Fgf2, Hif1a, Il1b, Il3, Il6, Il7, Ocln, Pf4, Tek (Tie2), Thbs1, Tnf, Tnfsf10 (Trail). | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC012 | Apoptosis qpcr array | Akt1, Birc3 (c-IAP1), Card6, Card9, Casp1 (Ice), Casp8 (Flice), Cd14, Fadd, Ifnb1, Ikbkb, Il12a, Il12b, Il1b, Il6, Irak1, Jun, Map3k7 (Tak1), Mapk1 (Erk2), Mapk8 (Jnk1), Mpo, Nfkb1, Nfkbia (IkBa/Mad3), Pik3ca, Prdx2, Pycard, Rac1, Ripk1, Ripk2, Tnf, Tnfrsf1a, Traf6. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC018 | Apoptosis qpcr array | Akt1, Apc, Bad, Bcl2, Cdh1 (E-Cadherin), Cdh13, Cdkn1A (p21Cip1/Waf1), Cdkn2a (p16Ink4), Gstp1,Igf1, Il6, Jun, Muc1, Nme1 (Nm23a), Rarb, Sfn (14-3-3s),Sfrp1, Tp53, Tp73, Twist1. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC046 | Apoptosis qpcr array | Abl1, Atm, Bard1, Bax, Bbc3, Brca1, Cdkn1a (p21Cip1/Waf1), Chek2 (Rad53), Csnk2a2, Ppp1r15a (Gadd34), Prkdc, Rad21, Rad9, Sirt1, Terf1, Tp53. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC055 | Apoptosis qpcr array | Anxa5, Bax, Bcl2, Bcl2l1 (Bcl-X), Casp1 (Ice), Casp3, Cav1, Ccl2 (Mcp-1), Ccl5 (Rantes), Cflar (Casper), Cx3cl1, Edn1, Ednra, Fas (Tnfrsf6), Faslg (Tnfsf6), Fgf2, Hif1a, Il1b, Il3, Il6, Il7, Ocln, Pf4, Ptk2, Tek (Tie2), Thbs1, Tnf, Tnfsf10 (Trail). | 300元/樣 |
檢測(cè)流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測(cè),檢測(cè)RNA純度及完整度,提供RNA質(zhì)量報(bào)告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實(shí)時(shí)定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計(jì)算PCR芯片中的各個(gè)Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。