運(yùn)用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時(shí)對(duì)某個(gè)信號(hào)通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測(cè),芯片上的基因包括了與研究對(duì)象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對(duì)不同的信號(hào)通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類(lèi)芯片,涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號(hào) | 名稱(chēng) | 基因列表 | 價(jià)格 |
啟因生物(wcgene.com) | HUMAN-WC010 | Cytokines and Chemokines qpcr array | BMP2, CCL15, CCL2, CSF3, CXCL11, CXCL12, CXCL13, CXCL14, CXCL3, CXCL5, CXCL6, CXCL9, IL10, IL6, IL8, PPBP, PTN, TNF. | 200元/樣 |
啟因生物(wcgene.com) | HUMAN-WC104 | Cytokines and Chemokines qpcr array | CCL11, CCL2 (MCP-1), CCL22 (MDC), CCL5 (RANTES), CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), CXCL1, CXCL10 (INP10), CXCL2, CXCL9 (MIG), FASLG (TNFSF6), IFNB1, IFNG, IL12B, IL15, IL1A, IL1B, IL1RN, IL2, IL4, IL6, IL8, LTA (TNFB), LTB, TNF, TNFSF10 (TRAIL). | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC103 | Cytokines and Chemokines qpcr array | Ccl12, Ccl22 (Mdc), Ccl5 (Rantes), Csf1 (MCSF), Csf2 (GM-CSF), Csf3 (GCSF), Cxcl1, Cxcl10 (Inp10), Cxcl3, Cxcl9 (Mig), Fasl (Tnfsf6), Ifnb1, Ifng, Il12b, Il15, Il1a, Il1b, Il1rn, Il2, Il4, Il6, Lta (Tnfb), Ltb, Tnf, Tnfsf10 (Trail). | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC105 | Cytokines and Chemokines qpcr array | Ccl11, Ccl12, Ccl22 (Mdc), Ccl5 (Rantes), Csf1 (MCSF), Csf2 (GM-CSF), Csf3 (GCSF), Cxcl1, Cxcl10 (Inp10), Cxcl3, Cxcl9 (Mig), Faslg (Tnfsf6), Ifnb1, Ifng, Il12b, Il15, Il1a, Il1b, Il1rn, Il2, Il4, Il6, Lta (Tnfb), Ltb, Tnf, Tnfsf10 (Trail). | 300元/樣 |
檢測(cè)流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測(cè),檢測(cè)RNA純度及完整度,提供RNA質(zhì)量報(bào)告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實(shí)時(shí)定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計(jì)算PCR芯片中的各個(gè)Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開(kāi)發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。