運(yùn)用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時(shí)對(duì)某個(gè)信號(hào)通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測(cè),芯片上的基因包括了與研究對(duì)象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對(duì)不同的信號(hào)通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號(hào) | 名稱 | 基因列表 | 價(jià)格 |
啟因生物(wcgene.com) | HUMAN-WC106 | Steroid Hormone Receptors qpcr array | ESRRA, ESRRB, ESRRG, HNF4A, NR0B1, NR0B2, NR1D1, NR1D2, NR1H2, NR1H3, NR1H4,NR1I2, NR2C1, NR2C2, NR2E3, NR2F1, NR2F2, NR2F6, NR3C2, NR4A1, NR5A1, NR6A1, PGR,PPARA, PPARD, PPARG, RARA, RARB, RARG, RORA, RXRA, RXRB, RXRG, THRA, THRB, VDR. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC105 | Steroid Hormone Receptors qpcr array | Esrra, Esrrb, Esrrg, Hnf4a, Nr0b1, Nr0b2, Nr1d1, Nr1d2, Nr1h2, Nr1h3, Nr1h4, Nr1i2, Nr2c1,Nr2c2, Nr2e3, Nr2f1, Nr2f2, Nr2f6, Nr3c2, Nr4a1, Nr5a1, Nr6a1, Pgr, Ppara, Ppard, Pparg,Rara, Rarb, Rarg, Rora, Rxra, Rxrb, Rxrg, Thra, Thrb, Vdr. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC107 | Steroid Hormone Receptors qpcr array | Esrra, Esrrb, Esrrg, Hnf4a, Nr0b1, Nr0b2, Nr1d1, Nr1d2, Nr1h2, Nr1h3, Nr1h4, Nr1i2,Nr2c1, Nr2c2, Nr2f1, Nr2f2, Nr2f6, Nr3c2, Nr4a1, Nr5a1, Nr6a1, Pgr, Ppara, Ppard, Pparg,Ppargc1a (PGC-1a), Ppargc1b (PGC-1β), Rara, Rarb, Rora, Rxra, Rxrb, Rxrg, Thra, Thrb, Vdr. | 300元/樣 |
檢測(cè)流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提取;
2. RNA質(zhì)量檢測(cè),檢測(cè)RNA純度及完整度,提供RNA質(zhì)量報(bào)告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實(shí)時(shí)定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計(jì)算PCR芯片中的各個(gè)Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。