顆粒轟擊用于體外和體內(nèi)基因轉(zhuǎn)移的優(yōu)點
* 簡單、快速、功能靈活的基因傳送，各種細胞類型通用
* 有助于瞬時表達和穩(wěn)定表達
* 只需少量的DNA 和細胞
* 無需載體DNA
* 能進行多質(zhì)粒共同導入
* 輸送大片段DNA
* 胞內(nèi)目標基因可導入多個細胞
* 用于體內(nèi)和體外轉(zhuǎn)化
* 沒有無關(guān)的基因或蛋白被轉(zhuǎn)導
The Helio gene gun is a convenient portable device for rapid and direct in-situ gene transfer of cells.The system USES an adjustable pulse of helium gas to drive gold particles of DNA, RNA or other biomaterials prepacked on the inside of a small plastic tube directly into a cell.The in-tube "bullet" can be easily prepared using the sample control station.
The advantages of particle bombardment for gene transfer in vitro and in vivo
* simple, fast, functional gene transfer across all cell types
* contributes to transient and stable expression
* only small amounts of DNA and cells are needed
* no carrier DNA is required
* multiple plasmids can be imported together
* transport large fragments of DNA
* target genes can be introduced into multiple cells
* for in vivo and in vitro transformation
* no unrelated genes or proteins are transduced
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