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BioXtal™(高通量)蛋白結(jié)晶條件篩選試劑盒

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  • 公司名稱博亞捷晶科技(北京)有限公司
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  • 更新時間2021/8/10 20:22:24
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博亞捷晶科技(北京)有限公司于2006年在北京市海淀區(qū)中關(guān)村留學歸國人員創(chuàng)業(yè)園內(nèi)成立,公司主要致力于為生命科學領(lǐng)域的研究提供服務和支持。 目前公司的業(yè)務主要集中在提供蛋白質(zhì)晶體培養(yǎng)所需的耗材、試劑與儀器。其中包括蛋白結(jié)晶條件篩選盒、晶體培養(yǎng)板、蛋白晶體生長情況觀測機器人。2018年初,博亞捷晶成為美國牛津?qū)嶒瀮x器(Oxford Lab Product)在中國設立的辦事處,產(chǎn)品涵蓋手動及電動移液器、離心機、渦旋振蕩器、磁力攪拌器、搖床、混勻儀和耗材等一系列產(chǎn)品。
移液器
產(chǎn)品詳情相比近年來有長足進展的生物樣品純化和晶體X射線衍射技術(shù),生物大分子晶體的培養(yǎng)依然是一個試驗探索的過程,極大程度地依賴于經(jīng)驗并帶有較大的盲目性
BioXtal™(高通量)蛋白結(jié)晶條件篩選試劑盒 產(chǎn)品信息

產(chǎn)品詳情

    相比近年來有長足進展的生物樣品純化和晶體X射線衍射技術(shù),生物大分子晶體的培養(yǎng)依然是一個試驗探索的過程,極大程度地依賴于經(jīng)驗并帶有較大的盲目性。 蛋白結(jié)晶所需的各種成分,如沉淀劑、鹽、緩沖液體系、有機溶劑以及各種添加劑,構(gòu)成一個龐大的多維集合。雖然現(xiàn)在市場上有一些商業(yè)化的晶體生長條件篩選盒,但它們只能構(gòu)成一個一個獨立的小子集, 遠遠無法覆蓋非常復雜、巨大的蛋白結(jié)晶條件集合。

    博亞的BioXtal™結(jié)晶條件篩選盒是為蛋白晶體生長條件篩選而特別開發(fā)的一個晶體生長條件組合。 其成分組成是以大量發(fā)表和未發(fā)表的結(jié)晶統(tǒng)計數(shù)據(jù)為基礎發(fā)展而成。它既包含離散矩陣篩選模式也包括系統(tǒng)性篩選模式,既含*效的結(jié)晶條件也含許多其它結(jié)晶條件篩選盒所未有的條件。 擴展了篩選范圍從而增大了發(fā)現(xiàn)結(jié)晶條件的幾率。

    該試劑盒有96個條件以適合96孔板自動化篩選設備,pH范圍涵蓋蛋白晶體最易生長范圍4.5-8.5。所使用的化學試劑來自國標*化學試劑公司(Fluka、Sigma-Aldrich等),并且每種溶液都經(jīng)過0.22μ慮膜過慮滅菌處理。96個條件分別密封在螺旋蓋試管中,每管不少于10毫升,可隨便使用。

      在最近進行的大量蛋白晶體篩選測試研究中,博亞BioXtal™結(jié)晶條件篩選盒曾結(jié)晶出了超過50%的可結(jié)晶蛋白。被認為是高效結(jié)晶條件組合,是一個對現(xiàn)有商業(yè)化的結(jié)晶條件篩選盒的有力補充和發(fā)展。

BioXtalTM結(jié)晶條件篩選試劑盒

 

 1.  0.9M (NH4)2SO4 ,0.1M Na-acetate pH4.5

 2. 0.9M (NH4)2SO4 ,0.02M HEPES pH7.5 ,0.7M NaNO3

 3. 1.5M (NH4)2SO4 ,0.1M MES pH6.5

 4. 1.5M (NH4)2SO4 ,0.1M Bis-tris pH7.0,

     0.7%(w/v) Octyl-β-D-glucopyranoside

 5. 1.5M (NH4)2SO4  ,0.1M Tris-HCl pH7.5,

     0.05M MgCl2

 6. 2.0M (NH4)2SO4  ,0.1M Na-citrate pH5.0,

     0.5M NaNO3

 7. 2.0M (NH4)2SO4 ,0.1M Bis-tris pH5.5

 8. 2.0M (NH4)2SO4  ,0.1M Bis-tris pH6.5

 9. 2.0M (NH4)2SO4 ,0.02M HEPES pH7.5

10. 2.0M (NH4)2SO4 ,0.1M Tris-HCl pH8.0,

     0.02M CaCl2 ,0.5% Octyl-β-D-glucopyranoside

11. 2.0M (NH4)2SO4 ,0.1M Tris-HCl pH8.5

12. 2.0M (NH4)2SO4

13. 2.88M (NH4)2SO4 ,0.1M Na-citrate pH5.0

14. 2.88M (NH4)2SO4 ,0.02M HEPES pH7.5

15. 15.0%(v/v) Jeffamine M600 ,0.1M HEPES pH7.0

16. 30.0%(w/v) Jeffamine ED-2001 ,0.1M HEPES pH7.0

17. 1.8M Li2SO4 ,0.05M Na Cacodylate pH6.0,

     0.01M MgSO4

18. 2.0M Li2SO4 ,0.05M MES pH5.6 ,0.01M MgCl2

19. 15.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH7.5 ,

     0.2M KCl

20. 20.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH8.5,

    0.2M trimethylamine n-oxide

21. 28.0%(w/v) PEG 2000 MME ,0.1M Bis-tris pH6.5

22. 30.0%(w/v) PEG 2000 MME ,

     0.05M Na Cacodylate pH6.0 ,0.05M MgCl2

23. 30.0%(w/v) PEG 2000 MME ,0.15M KBr

24. 20.0%(w/v) PEG 5000 MME ,0.1M Bis-tris pH6.5

25. 20.0%(w/v) PEG 5000 MME ,0.1M HEPES pH7.0,

     0.5M NaNO3

26. 25.0%(w/v) PEG 5000 MME ,0.1M Tris-HCl pH7.5

27. 25.0%(v/v) PEG 550 MME ,0.1M Na-citrate pH5.0

28. 25.0%(v/v) PEG 550 MME ,0.1M Tris-HCl pH8.5

29. 30.0%(v/v) PEG 550 MME ,0.1M Bis-tris pH7.0 ,

     0.05M MgCl2

30. 10.0%(v/v) MPD ,0.1M Na-citrate pH5.0

31. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 0.1M CaCl2

32. 20.0%(v/v) MPD ,0.05M Na Cacodylate pH6.0

33. 25.0%(v/v) MPD ,0.1M Na-citrate pH5.0

34. 25.0%(v/v) MPD ,0.1M MES pH6.5 ,0.75M NaNO3

35. 25.0%(v/v) MPD ,0.02M HEPES pH7.5 ,0.05M MgCl2

36. 30.0%(v/v) MPD ,0.1M Na-acetate pH4.5

37. 30.0%(v/v) MPD ,0.1M MES 6.5 ,0.02M CaCl2

38. 30.0%(v/v) MPD ,0.1M Tris-HCl pH8.5 ,0.02M MgCl2

39. 40.0%(v/v) MPD ,0.01M CaCl2

40. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 ,0.01M MgCl2 ,

     0.05M NH4-acetate

41. 1.2M NaCl ,0.1M MES pH6.5

42. 1.2M NaCl ,0.6M NaNO3 ,

     0.25%(w/v) Octyl-β-D-glucopyranoside

43. 1.5M NaCl ,0.1M Na-acetate pH4.5

44. 1.5M NaCl ,0.1M Bis-tris pH7.0

45. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.01M CaCl2 ,

     0.4%(w/v)  Octyl-β-D-glucopyranoside

46. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.6M NaNO3

47. 1.5M NaCl 0.1M NH4-acetate ,

     0.4% Octyl-β-D-glucopyranoside

48. 2.0M NaCl ,0.02M HEPES 7.5

49. 2.0M NaCl ,0.3M NaNO3

50. 2.5M NaCl ,0.1M Na-citrate pH5.0 ,

     0.9M NaNO3

51. 2.5M NaCl ,0.1M Bis-tris pH6.5 ,0.3M NaNO3

52. 2.5M NaCl ,0.1M Bis-tris pH7.0 ,

     0.1M NH4-acetate

 

53. 2.5M NaCl ,0.02M HEPES pH7.5 ,0.05M MgCl2

54. 2.5M NaCl ,0.02M HEPES pH7.5

55. 2.9M NaCl ,0.1M Tris-HCl pH8.5,

     0.4%(w/v) Octyl-β-D-glucopyranoside

56. 3.0M NaCl ,0.1M Na-citrate pH5.0 ,0.5M NaNO3

57. 3.1M NaCl ,0.1M Bis-tris pH7.0

58. 3.5M NaCl ,0.1M Bis-tris pH5.5

59. 3.5M NaCl ,0.1M Tris-HCl pH7.5 ,0.05M MgCl2

60. 30.0%(w/v) PEG 1000 ,0.1M Tris-HCl pH7.5 ,

     5.0%(v/v) Glycerol

61. 17.0%(w/v) PEG 10000 ,0.1M Bis-tris pH5.5 ,

     0.1M NH4-acetate

62. 15.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.85M NaNO3

63. 17.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5

64. 20.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5 ,

     0.05M MgCl2

65. 20.0%(w/v) PEG 3350 ,0.2M Na-formate

66. 21.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5,

     0.7% Octyl-β-D-glucopyranoside

67. 22.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.08M CaCl2 ,

     0.7% Octyl-β-D-glucopyranoside

68. 24.0%(w/v) PEG 3350 ,0.01M CaCl2

69. 25.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

70. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 ,

     0.2M Li2SO4

71. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5,

     0.2M NaCl

72. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5

73. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5,

     0.2M NH4-acetate

74. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 ,

    0.2M MgCl2

75. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 ,

    0.2M MgCl2

76. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 ,

     0.2M NH4-acetate

77. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,

     0.2M MgCl2

78. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,0.2M NaCl

79. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5

80. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,

     0.2M NH4-acetate

81. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5,

     0.2M (NH4)2SO4

82. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,   

    0.2M NaCl

83. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5

84. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,

     0.2M NH4-acetate

85. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,

     0.2M MgCl2

86. 28.0%(w/v) PEG 3350 ,0.1M Bis-tris pH 7.0 ,

     0.02M CaCl2

87. 30.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

88. 30.0%(w/v) PEG 3350 ,0.4M NaNO3

89. 40.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

90. 40.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH7.5 ,

     0.4M NaNO3

91. 30.0%(v/v) PEG 400 ,0.05M Na Cacodylate pH6.0

92. 30.0%(v/v) PEG 400 ,0.1M HEPES pH7.5 ,0.2M MgCl2

93. 35.0%(v/v) PEG 400 ,0.1M Bis-tris pH6.5 ,

     0.1M NH4-acetate

94. 25.0%(w/v) PEG 4000 ,0.1M Na-acetate pH4.6 ,

     0.2M (NH4)2SO4

95. 30.0%(v/v)  pentaerythritol ethoxylate  (15/4 EO/OH) ,

     0.05M Bis-tris pH6.5  ,0.05M (NH4)2SO4

96. 35.0%(v/v) pentaerythritol propoxylate (5/4 PO/OH) ,

     0.05M HEPES pH7.5 ,0.2M KCl

 

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