RN38-EASYspin Plus 植物RNA快速提取試劑盒
注：本產(chǎn)品僅用于科研，不可用于臨床
貨號# | 規(guī)格 | 價格 |
RN3802 | 50 次 | 910 元 |
產(chǎn)品介紹
本公司推出EASYspin無苯fen、氯仿RNA快速提取技術(shù)基礎(chǔ)上，又成功研發(fā)基因組DNA清除柱技術(shù)可以有效清除gDNA殘留，得到的RNA一般不需要DNase消化，可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。裂解液迅速裂解細(xì)胞和滅活細(xì)胞RNA酶，植物RNA助提劑PLANTaid幫助結(jié)合多糖多酚并通過離心去除，然后裂解混合物用乙醇調(diào)節(jié)RNA結(jié)合吸附到基因組DNA清除柱，基因組DNA清除柱子同時吸附清除殘留的DNA, 然后RNA被選擇性洗脫濾過。濾過的RNA用乙醇調(diào)節(jié)結(jié)合條件后，RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜， 再通過一系列快速的漂洗－離心的步驟, 去蛋白液和漂洗液將細(xì)胞代謝物，蛋白等雜質(zhì)去除， 最后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點：
1.不使用有毒的苯fen，氯仿等試劑，也不需要乙醇沉淀等步驟。
2.簡捷，單個樣品操作一般可在25分鐘內(nèi)完成，簡單快速的試劑盒。
3.植物RNA助提劑可以有效結(jié)合多糖多酚，提高清除效果。
4.成功研發(fā)基因組DNA清除柱技術(shù)可以有效清除gDNA殘留，得到的RNA一般不需要DNase消化，可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。
5.適應(yīng)性極其廣泛，可以提取包括棉花、松針、冬青樹葉、葡萄葉片、等100多種國內(nèi)外試劑盒提取失敗的樣品。詳細(xì)樣品列表請參考公司主頁產(chǎn)品介紹。
6.多次柱漂洗確保高純度，OD260/OD280典型的比值達(dá)1.9～2.2，基本無DNA殘留,可用于RT-PCR，Northern-blot和各種實驗。
使用RN38-EASYspin Plus 植物RNA快速提取試劑盒 發(fā)表的部分文獻(xiàn)?。?！
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注：本產(chǎn)品僅用于科研，不可用于臨床
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