Mito-TEMPO
Mito-TEMPO 是一種靶向線粒體的超氧化物歧化酶模擬物，有著清chu超氧化物和烷基自由基的能力。
生物活性
Mito-TEMPO is a mitochondria-targeted superoxide dismutase mimetic with superoxide and alkyl radical scavenging properties[1].
體內(nèi)研究(In Vivo)
Mito-TEMPO (MT) greatly attenuates the increase in ALT activities and reduces the areas of necrosis at both time points, indicating that the protection by Mito-TEMPO is sustained until at least 24 h post-APaP. Mito-Tempo could induce secondary apoptosis in the late phase of APaP hepatotoxicity. Mito-Tempo induces secondary apoptosis after APaP overdose by inhibition of RIP3[1].
分子量：510.03
性狀：Solid
Formula：C29H35ClN2O2P
CAS 號：1334850-99-5
運輸條件
Room temperature in continental US; may vary elsewhere.
儲存方式
-20°C, sealed storage, away from moisture
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶解性數(shù)據(jù)
DMSO : 125 mg/mL (245.08 mM; Need ultrasonic)
H2O : 60 mg/mL (117.64 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9607 mL | 9.8033 mL | 19.6067 mL |
| 5 mM | 0.3921 mL | 1.9607 mL | 3.9213 mL |
| 10 mM | 0.1961 mL | 0.9803 mL | 1.9607 mL |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 儲存時，請在 6 個月內(nèi)使用，-20°C 儲存時，請在 1 個月內(nèi)使用。
以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶
- 1.
請依序添加每種溶劑： PBS
Solubility: 50 mg/mL (98.03 mM); Clear solution; Need ultrasonic
- 2.
請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.25 mg/mL (4.41 mM); Clear solution
- 3.
請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.25 mg/mL (4.41 mM); Clear solution
- 4.
請依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2.25 mg/mL (4.41 mM); Clear solution
參考文獻
[1]. Du K, et al. Mito-tempo protects against acute liver injury but induces limited secondary apoptosis during the late phase of acetaminophen hepatotoxicity. Arch Toxicol. 2018 Oct 15. [Content Brief]
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