iFSP1
iFSP1 是 FSP1 (AIFM2) 的選擇性有效抑制劑，EC50 值為 103 nM。iFSP1 可選擇性地誘導(dǎo)過表達(dá) FSP1 的 GPX4 敲除細(xì)胞發(fā)生鐵死亡 (ferroptosis)。iFSP1 可增強(qiáng)多種人ai細(xì)胞系對(duì)鐵死亡誘導(dǎo)劑的敏感度，如: (1S,3R)-RSL3 (HY-100218A)。
生物活性
iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1) (AIFM2) with an EC50 of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout cells which overexpressed FSP1. iFSP1 is able to sensitize a variety of human cancer cell lines to the ferroptosis inducer, such as (1S,3R)-RSL3 (HY-100218A)[1].
IC50 & Target
EC50: 103 nM (FSP1)[1]
體外研究(In Vitro)
iFSP1 (0.001-1 μM; 24 hours) inhibits the Gpx4-knockout cell growth as a dose-dependent manner, but does not inhibit the wild type cell growth. Treatment with the ferroptosis inhibitor Lip-1 protects GPX4- knockout cells from iFSP1-induced ferroptosis[1].
iFSP1 (0.001-1 μM; 24 hours) is less efficient than genetic deletion of FSP1, whereas iFSP1 treatment in the FSP1-knockout background had no additive effect to RSL3-induced ferroptosis[1].
iFSP1 (3 μM; 24 hours) treatment results in an obvious toxicity of RSL3 in a panel of genetically engineered (FSP1-knockout) human cancer cell lines[1].
AIFM2: the flavoprotein apoptosis-inducing factor mitochondria-associated 2 is a previously unrecognized anti-ferroptotic gene. AIFM2, which is renamed ferroptosis suppressor protein 1 (FSP1)[1]
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay[1]
| Cell Line: | Wild-type and Gpx4-knockout Pfa1 or HT 1080 cells overexpressing FSP1–HA |
| Concentration: | 0.001-1 μM |
| Incubation Time: | 24 hours |
| Result: | Was toxic to cells that depend solely (no GPX4 expression detectable) on FSP1 function. |
分子量：323.35
性狀：Solid
Formula：C20H13N5
CAS 號(hào)：150651-39-1
運(yùn)輸條件
Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數(shù)據(jù)
DMSO : 20 mg/mL (61.85 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.0926 mL | 15.4631 mL | 30.9262 mL |
| 5 mM | 0.6185 mL | 3.0926 mL | 6.1852 mL |
| 10 mM | 0.3093 mL | 1.5463 mL | 3.0926 mL |
請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)月內(nèi)使用。
以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶
- 1.
請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2 mg/mL (6.19 mM); Suspended solution; Need ultrasonic
參考文獻(xiàn)
[1]. Doll S, et al. FSP1 is a glutathione-independent ferroptosis suppressor.Nature. 2019 Nov;575(7784):693-698. [Content Brief]
注：產(chǎn)品僅用于科研
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