甲基丙胺
Methylproamine 是 DNA結合的輻射保護劑，它通過修復短暫輻射誘導的 DNA 氧化物種起作用，也可通過保護 DNA 雙鏈斷裂來防止電離輻射。
生物活性
Methylproamine is a DNA-binding radioprotector, acts by repair of transient radiation-induced oxidative species on DNA. Methylproamine also protects against ionizing radiation by preventing DNA double-strand breaks[1].
體外研究(In Vitro)
Methylproamine also protects against ionizing radiation by preventing DNA double-strand breaks[1].
Methylproamine can protect bystander cells from radiation-induced DNA damage[2].
Methylproamine has a concentration-dependent radioprotective effect[3]
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Cytotoxicity Assay[3]
| Cell Line: | Keratinocytes |
| Concentration: | 10, 20 μM |
| Incubation Time: | 60 min |
| Result: | Did not show any detectable cytotoxicity at 10 μM and had appreciable cytotoxicity at 20 μM. |
分子量：465.59
Formula：C28H31N7
CAS 號：188247-01-0
運輸條件：Room temperature in continental US; may vary elsewhere.
儲存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數(shù)據(jù)
DMSO : ≥ 41 mg/mL (88.06 mM)
* "≥" means soluble, but saturation unknown.
| 1 mM | 2.1478 mL | 10.7391 mL | 21.4781 mL |
| 5 mM | 0.4296 mL | 2.1478 mL | 4.2956 mL |
| 10 mM | 0.2148 mL | 1.0739 mL | 2.1478 mL |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 儲存時，請在 6 個月內使用，-20°C 儲存時，請在 1 個月內使用。
請根據(jù)您的實驗動物和給藥方式選擇適當?shù)娜芙夥桨?。以下溶解方案都請先按?In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶
- 1.
請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 0.62 mg/mL (1.33 mM); Clear solution
- 2.
請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 0.62 mg/mL (1.33 mM); Clear solution
參考文獻
[1]. Carl N Sprung, et al. Methylproamine protects against ionizing radiation by preventing DNA double-strand breaks. [Content Brief]
[2]. Susanne Burdak-Rothkamm, et al. Radioprotection of targeted and bystander cells by methylproamine. Strahlenther Onkol. 2015 Mar;191(3):248-55. [Content Brief]
[3]. Pavel N Lobachevsky, et al. Protection by methylproamine of irradiated human keratinocytes correlates with reduction of DNA damage. Int J Radiat Biol. 2011 Mar;87(3):274-83. [Content Brief]
[4]. Lobachevsky PN, Vasireddy RS, Broadhurst S, Protection by methylproamine of irradiated human keratinocytes correlates with reduction of DNA damage. Int J Radiat Biol. 2011 Mar;87(3):274-83. [Content Brief]
[5]. Sprung CN, Vasireddy RS, Karagiannis TC, Methylproamine protects against ionizing radiation by preventing DNA double-strand breaks. Mutat Res. 2010 Oct 13;692(1-2):49-52. [Content Brief]
[6]. Martin RF, Broadhurst S, Reum ME, In vitro studies with methylproamine: a potent new radioprotector. Cancer Res. 2004 Feb 1;64(3):1067-70. [Content Brief]
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