七葉苷培養(yǎng)基復(fù)蘇菌種前應(yīng)準(zhǔn)各適于該菌種生長(zhǎng)的固體、液體培養(yǎng)基和培養(yǎng)設(shè)備,所有操作均應(yīng)在符合生物安全保護(hù)及無(wú)菌條件下進(jìn)行。
啟開(kāi)菌種宜采用鋸開(kāi)法(鋸開(kāi)前用75%酒精棉消毒菌種管外壁)。用吸管將液體培養(yǎng)基0.3ml左右注入被啟開(kāi)的菌種安瓶中并吹打,使安瓶中的凍干菌種溶解成菌懸液。
將上述菌懸液全部吸出,分別接種到固體斜面和液體培養(yǎng)管中,一般放置在37℃培養(yǎng)箱培養(yǎng)18~24小時(shí)。對(duì)需要特殊培養(yǎng)條件的細(xì)菌,如需5%~10%CO2或厭氧條件等,應(yīng)分別于CO2培養(yǎng)箱或厭氧培養(yǎng)箱中培養(yǎng)。
七葉苷培養(yǎng)基產(chǎn)品說(shuō)明:
是一類(lèi)利用微生物自身代謝產(chǎn)生的酶與相應(yīng)顯色底物反應(yīng)顯色的原理來(lái)檢測(cè)微生物的新型培養(yǎng)基。
這些相應(yīng)的顯色底物是由產(chǎn)色基因和微生物部分可代謝物質(zhì)組成,在特異性酶作用下,游離出產(chǎn)色基因顯示一定顏色,直接觀察菌落顏色即可對(duì)菌種作出鑒定。
它是一種新型分離培養(yǎng)基,利用顯色培養(yǎng)基進(jìn)行微生物的篩選分離,其反應(yīng)的靈敏度和特異性大大優(yōu)于傳統(tǒng)培養(yǎng)基。
RC-1175R Anti-SHBG (Sex Hormone Binding Globulin) 性激素結(jié)合球蛋白抗體
RC-0882R Shiga-like toxin IIe variant subunit A[Escherichia coli] 抗豬水腫病大腸桿菌志賀樣毒素Ⅱ型突變體(O139菌株)抗體
RC-1129R Anti-SHP-1 (Src homology phosphatase-1) 造血細(xì)胞磷酸酶抗體
RC-1233R Anti-SHP2/PTPN11(Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2) 蛋白酪氨酸磷酸酶2抗體
RC-1130R Anti-SIP1 (survival of motor neuron protein interacting protein 1) 運(yùn)動(dòng)神經(jīng)元存活蛋白結(jié)合蛋白1抗體
RC-1096R Anti-Skp2 (S-phase kinase-associated protein 2,SKP2) 細(xì)胞S相激酶相關(guān)蛋白抗體
c Anti-Slc22A17 (solute carrier famili 22A17) 可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白22A17抗體
RC-0496R Anti-SLC24A5 (solute carrier family 24, member 5) 可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白SLC24A5抗體
RC-0478R Anti-SLC6A19 (Solute carrier family 6, member 19) 鉀依賴鈉鈣交換蛋白6
RC-1298R anti-Smac/DIABLO(the second mitochondrial activator of caspase) 線粒體促凋亡蛋白抗體
RC-0856R SLC34A2/NaPi-2b(Solute carrier family 34 member 2) 磷酸鈉協(xié)同轉(zhuǎn)運(yùn)蛋白抗體
RC-0718R Anti-Smad2/3(Mothers against decapentaplegic homolog 2) Smad 2/3抗體
RC-0585R Anti-Smad4(Mothers against decapentaplegic homolog 4) Smad4抗體
RC-0566R Anti-Smad7(Mothers against decapentaplegic homolog 7) Smad 7抗體
RC-0259R Anti-SM-MHC(Cardiac myosin heavy chain) 心肌肌凝蛋白抗體
RC-0602R Anti-Snake poison Protein 抗蛇毒蛋白抗體(蝮蛇)
RC-0763R Anti-Snake poison Protein 抗蛇毒蛋白抗體(中華眼鏡蛇)
RC-1131R Anti-SNAP-25 (synaptosomal-associated protein 25) 突觸相關(guān)膜蛋白25抗體
RC-0113R Anti-Socs 1 (suppressor of cytokine signaling 1) 細(xì)胞因子信號(hào)傳導(dǎo)抑制蛋白1抗體
RC-0580R Anti-Socs 3 (suppressor of cytokine signaling 3) 細(xì)胞因子信號(hào)傳導(dǎo)抑制蛋白3抗體
RC-1079R Anti-SOD1 (superoxide-dimutase-1) 超氧化物歧化酶1抗體
RC-1080R Anti-SOD2 (superoxide-dimutase-2) 超氧化物歧化酶2抗體
RC-0473R Anti-Solute carrier family 1 溶質(zhì)攜帶物家族-1抗體
RC-1132R Anti-Somatostatin/GRIH 生長(zhǎng)抑素抗體
RC-0523R Anti-Sox2 胚胎干細(xì)胞關(guān)鍵蛋白抗體
RC-0065R Anti-SP (SuRCtance P) P物質(zhì)抗體
RC-0770R Anti-SP-A (pulmonary surfactant-associated glycoprotein A) 肺表面活性蛋白A抗體