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RN5301
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杭州市
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RN53-EASYspin Plus 多糖多酚/復(fù)雜植物RNA快速提取試劑盒
注:本產(chǎn)品僅用于科研,不可用于臨床
貨號# | 規(guī)格 | 價格 |
RN5301 | 50 次 | 1580.00 元 |
產(chǎn)品介紹:
本公司推出EASYspin無苯fen、氯仿RNA快速提取技術(shù)基礎(chǔ)上,又研發(fā)成功基因組DNA清除柱技術(shù)可以有效清除gDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。裂解液迅速裂解細胞和滅活細胞RNA酶, 離心沉淀去除多糖多酚和次級代謝產(chǎn)物,然后裂解混合物用乙醇調(diào)節(jié)RNA結(jié)合吸附到基因組DNA清除柱,然后RNA被選擇性洗脫濾過, 吸附在基因組DNA清除柱上的殘留DNA無法洗脫連同柱子一起丟棄從而清除掉DNA。濾過的RNA用乙醇調(diào)節(jié)結(jié)合條件后,RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜,再通過一系列快速的漂洗-離心的步驟,去蛋白液和漂洗液將細胞代謝物,蛋白等雜質(zhì)去除, 最后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點:
1.不使用有毒的苯fen,氯仿等試劑,也不需要乙醇沉淀等步驟。
2.簡捷,單個樣品操作一般可在25分鐘內(nèi)完成,快速的試劑盒。
3.成功研發(fā)基因組DNA清除柱技術(shù)可以有效清除gDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。
4.適應(yīng)性極其廣泛,可以提取包括復(fù)雜中草藥如石斛/丹參/雪蓮/人參、復(fù)雜淀粉種子如水稻/小麥/玉米種子、復(fù)雜果實如葡萄/藍莓/草莓/西瓜果實、復(fù)雜抗逆植物如冬青/松針/沙棘/胡楊、復(fù)雜花卉月季/玫瑰/梅/牡丹花、復(fù)雜多糖植物紫菜/仙人掌/蘆薈水稻種子等數(shù)百種國內(nèi)外試劑盒提取失敗的樣品,使用EASYspin系列植物RNA提取試劑盒累計發(fā)表文章超過200篇。詳細樣品列表請參考公司主頁產(chǎn)品介紹或者找我們索取200多篇發(fā)表文章原文。
5.多次柱漂洗確保高純度,OD260/OD280典型的比值達1.9~2.2,無DNA殘留,可用于熒光定量PCR、RT-PCR、芯片、二代測序、Northern-blot等各種實驗。
使用RN53多糖多酚/復(fù)雜植物RNA快速提取試劑盒 發(fā)表部分文獻
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注:本產(chǎn)品僅用于科研,不可用于臨床
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