說(shuō)明書(shū)下載EATP.rar

分類名稱 : 代謝產(chǎn)物分析 產(chǎn)品介紹 :

產(chǎn)品說(shuō)明  三磷酸腺苷(ATP)是細(xì)胞代謝的化學(xué)能量，常被稱為細(xì)胞的“能源貨幣”。ATP只在活細(xì)胞進(jìn)行光合作用和呼吸作用時(shí)產(chǎn)生，在細(xì)胞生命活動(dòng)包括生物合成反應(yīng)、細(xì)胞運(yùn)動(dòng)和細(xì)胞分裂過(guò)程中消耗。它是細(xì)胞活動(dòng)的重要指標(biāo)，并且在研究和藥物研發(fā)中已被用作一種細(xì)胞活性和細(xì)胞毒性的檢測(cè)方法。本公司生產(chǎn)的ATP檢測(cè)試劑盒為測(cè)量細(xì)胞內(nèi)的ATP提供了一種快速的方法。用專門(mén)的工作試劑將細(xì)胞溶解，釋放出ATP，ATP在熒光素酶的作用下，立即與底物D-熒光素反應(yīng)產(chǎn)生光。光的強(qiáng)度可直接用來(lái)測(cè)量細(xì)胞內(nèi)ATP濃度。這種非放射性、均相的細(xì)胞檢測(cè)在微孔板中進(jìn)行。該試劑適用于所有培養(yǎng)基和高通量藥物篩選中應(yīng)用的96孔板、384孔板和液體加樣設(shè)備。

產(chǎn)品特點(diǎn) 安全: 無(wú)放射性檢測(cè)。靈敏、準(zhǔn)確: zui低檢測(cè) 0.1 mM ATP 或 40個(gè)細(xì)胞。均相、方便: 混合-檢測(cè)型”的均相檢測(cè)方式。不涉及清洗和試劑轉(zhuǎn)移的步驟。高通量: 在96孔板和384孔板中常觀察到Z’ 因子>0.5。采用自動(dòng)化的液體加樣設(shè)備，每天可處理上千上萬(wàn)個(gè)樣本。

產(chǎn)品用途 細(xì)胞和其他生物樣品中ATP的測(cè)定。

試劑盒規(guī)格  10 mL 緩沖液, 120 uL 底物試劑, 5 mL ATP酶, 100 uL 3 mM ATP 標(biāo)準(zhǔn)品。

儲(chǔ)藏條件  冰上運(yùn)輸，-20°C 保存。保質(zhì)期6個(gè)月。

Description  Adenosine 5-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. Our ATP Assay Kit provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediay reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

Key features  Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1 mM ATP or 40 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z’ factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Applications   ATP determination in cells and other biological samples.

Kit Contents    10 mL Assay Buffer, 120 uL Substrate, 5 uL ATP Enzyme, 100 uL Standard.

Storage conditions   store all reagents at -20°C. This kit is shipped on ice. Shelf life of at least 6 months.

編號(hào)：EATP-100

測(cè)試：100T