說明書下載ECET.rar

分類名稱 : 血液尿液化學(xué),代謝產(chǎn)物分析 產(chǎn)品介紹 :

測定方法     比色法（565nm）定量測定乙醇的濃度。

產(chǎn)品說明     含酒精的飲料屬于日常飲用的飲料。研究表明，酗酒可能導(dǎo)致各種形式的肝臟疾病和促進死亡率的上升。定量測定酒精(乙醇，C₂H₅OH)濃度被廣泛應(yīng)用在基礎(chǔ)研究，藥物開發(fā)，臨床研究和酒類制造業(yè)。簡單，直接，自動化測量乙醇濃度方法是非?？扇〉摹２┦郎锛夹g(shù)公司的乙醇測試盒是基于乙醇脫氫酶催化氧化乙醇，其中形成的NADH耦合到甲臜(MTT)發(fā)色體上。產(chǎn)物的顏色強度(565nm)與樣品中乙醇的濃度成正比。

適用范圍     直接檢測：生物樣品如血清、血漿、尿液、唾液中的酒精。藥理學(xué): 藥物對酒精代謝的影響。

產(chǎn)品特點     靈敏、準(zhǔn)確: 檢測下限為0.0008 vol % (140 mM or 8 ppm),線性度高達0.1%酒精(96-孔板法)。使用方便：僅添加單一工作試劑，培養(yǎng)30分鐘，不需加熱器。高通量篩選：通過96-孔板高通量的自動化檢測，每天可以檢測上千份樣品。

貯藏條件：室溫下儲存終止液，其他所有試劑均于-20°C保存。

Description     Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C₂H₅OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. our  EnzyChrom^TM ethanol assay kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample.

Applications     Direct Assays: ethanol in serum, plasma, urine and saliva samples. Pharmacology: effects of drugs on alcohol metabolism.

Key features     Sensitive and accurate. Detection limit 0.0008 vol % (140 mM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay.Convenient. The procedure involves adding working reagent, incuba-ting for 30 min and stropping reaction. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Storage conditions. Store Stop Reagent at room temperature and all other reagents at -20°C. Shelf life of at least 6 months.

編號：ECET-048, ECET-100

測試：48T, 100T