說明書下載ENH3.rar

分類名稱 : 高效藥物篩選,代謝產(chǎn)物分析 產(chǎn)品介紹 :

測定方法    比色法(340nm)或熒光法(450/360nm)測定氨濃度

產(chǎn)品說明   對生命機體來說氨(NH₃)或銨離子形式(NH₄⁺)是氮的重要來源。它是通過氨基酸代謝合成，當呈現(xiàn)高濃度時則有毒性。在肝臟，氨通過尿素循環(huán)轉(zhuǎn)換成尿素。血液中氨濃度升高(高血氨癥)多見于肝功能障礙(肝硬化)患者，而氨濃度低下則與尿素循環(huán)酶(如鳥氨酸轉(zhuǎn)移酶)障礙相關(guān)。簡單，直接，自動化的氨濃度檢測手段在科研和藥物開發(fā)領(lǐng)域是很受歡迎的。博世生物技術(shù)的氨濃度檢測試劑盒是直接檢測NH₃和NH₄⁺的濃度。檢測中，NADH在氨、戊二酸和谷氨酸脫氫酶的參與下被轉(zhuǎn)換為NAD⁺ 。在340 nm處光密度或在lem/ex  =  450/360nm處熒光強度與樣品中氨的濃度成正比。

產(chǎn)品特點   靈敏度高、線性范圍寬: 僅用20 mL的樣本。線性檢測范圍24-1000 mM氨.操作簡單: 簡單的“混合-檢測” 操作, 可在30分鐘內(nèi)檢測氨濃度。

適用范圍   直接檢測：生物樣品(如血清，血漿，尿液， 唾液，細胞培養(yǎng)等)中的氨。

貯存條件:  該試劑盒應(yīng)冰運, 所有試劑均于-20°C保存。 

Description    Ammonia (NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).Simple, direct and automation-ready procedures for measuring NH₃ are popular in research and drug discovery. our  ammonia assay is designed to directly measure NH₃ and NH₄⁺. In this assay, NADH is converted to NAD⁺ in the presence of NH₃, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at l_em/ex = 450/360 nm is directly proportionate to the NH₃ concentration in the sample.

Key features   High sensitivity and wide linear range. Use 20 mL sample. Linear detection range 24 to 1000 mM ammonia.Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of NH₃ within 30 minutes.

Applications    Direct Assays: NH₃ in biological samples (e.g. serum, plasma, urine, saliva, cell culture etc).

Storage conditions. The kit is shipped chilled.  Store all components at -20°C. Shelf life of at least 3 months.

編號：ENH3-048，ENH3-100

測試：48T,100T