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高遷移率族蛋白B1抗體HMGB1英文名稱：HMGB1
中文名稱：高遷移率族蛋白B1抗體
別    名：Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; High mobility group protein B1; HMG3; HMGB 1;HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.
研究領(lǐng)域：腫瘤  細(xì)胞生物  免疫學(xué)  轉(zhuǎn)錄調(diào)節(jié)因子  結(jié)合蛋白  
抗體來(lái)源：Rabbit
克隆類型：Polyclonal
交叉反應(yīng)：Human, Mouse, Rat, Cow, 
產(chǎn)品應(yīng)用：WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量：24kDa
細(xì)胞定位：細(xì)胞核 
性    狀：Lyophilized or Liquid
濃    度：1mg/1ml
免 疫 原：KLH conjugated synthetic peptide derived from human HMGB1
亞    型：IgG
純化方法：affinity purified by Protein A
儲(chǔ) 存 液：0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

產(chǎn)品介紹：background:

High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models 

Function:
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J rebination by acting as a cofactor of the RAG plex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved rebination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).
Subunit:
Component of the RAG plex posed of core ponents RAG1 and RAG2, and associated ponent HMGB1 or HMGB2 (By similarity).
Subcellular Location:
Nucleus. Chromosome. 
Similarity:
Belongs to the HMGB family. 
Contains 2 HMG box DNA-binding domains.

Gene ID:
3146
Database links:
Entrez Gene: 282691 Cow
Entrez Gene: 3146 Human
Entrez Gene: 100862258 Mouse
Entrez Gene: 15289 Mouse
Entrez Gene: 25459 Rat
Omim: 163905 Human
SwissProt: P10103 Cow
SwissProt: P09429 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
近來(lái)的研究表明稱之為高遷移率族蛋白B-1（HMG-B1）的核內(nèi)結(jié)構(gòu)蛋白在核外表達(dá)時(shí)是一種有效的早期炎癥介質(zhì)。
高遷移性Ｂ１組蛋白（ＨＭＧＢ１）： 是一種核結(jié)合蛋白，在ＤＮＡ重組、修復(fù)、復(fù)制和基因轉(zhuǎn)錄中起作用。ＨＭＧＢ１也是巨噬細(xì)胞分泌的一種介質(zhì)。
此外，高遷移性Ｂ１組蛋白亦被受刺激的巨噬細(xì)胞或單核細(xì)胞主動(dòng)分泌。在這一主動(dòng)分泌過(guò)程中，HMGB1首先經(jīng)乙?；⒂珊藘?nèi)轉(zhuǎn)移溶酶體內(nèi)，繼而在ATP和溶血磷脂膽堿兩種分泌信號(hào)指導(dǎo)下轉(zhuǎn)移細(xì)胞外。由壞死細(xì)胞被動(dòng)釋放的HMGB1和炎癥細(xì)胞主動(dòng)分泌的HMGB1存在分子上的差異。胞外的HMGB1可作為細(xì)胞因子參與信號(hào)傳導(dǎo)，因?yàn)樗瓤勺R(shí)別Toll 樣受體（TLR）家族的一些成員，又能與識(shí)別晚期糖基化終末產(chǎn)物受體（RAGE）相作用。
HMGB1能啟動(dòng)炎癥反應(yīng)，包括產(chǎn)生多種細(xì)胞因子、對(duì)某些干細(xì)胞產(chǎn)生趨化作用、誘導(dǎo)血管粘附分子、削弱腸上皮細(xì)胞的功能等。
Protein: line1,mo macrophages lysates, 30ug; 
Primary: Anti-HMGB1 (bs-0664R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000; 
ECL excitated the fluorescence; 
Predicted band size : 24kD
Observed band size : 24kD