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人一氧化氮含量試劑盒(NO)齊一生物科技(上海)有限公司檢測試劑盒產(chǎn)品已被廣泛應(yīng)用于化學(xué),化工,生命科學(xué)的基礎(chǔ)研究和開發(fā)應(yīng)用,制藥,疾病診斷與控制,人口與健康,生物技術(shù)等諸多領(lǐng)域.客戶遍布國內(nèi)各大學(xué),研究所,醫(yī)院,衛(wèi)生防疫,商品檢驗(yàn)檢疫,制藥公司,生物技術(shù)公司和食品工業(yè)等單位.
人一氧化氮含量試劑盒(NO)elisa技術(shù)流程
ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記.結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性,酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性.在測定時,受檢標(biāo)本(測定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應(yīng).用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開.再加入酶標(biāo)記的抗原或抗體,也通過反應(yīng)而結(jié)合在固相載體上.此時固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例.加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析.由于酶的催化效率很高,間接地放大了免疫反應(yīng)的結(jié)果,使測定方法達(dá)到很高的敏感度.ELISA可用于測定抗原,也可用于測定抗體.
然而,影響Elisa試驗(yàn)結(jié)果的因素很多,故加強(qiáng)各個環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點(diǎn).
試驗(yàn)原理
試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.
Anti-ER-α/β Estrpgen Receptor α/β 雌激素受體α/β抗體
Anti-ER-β (Estrogen receptor-β) 雌激素受體β 抗體
Anti-ER-β (Estrogen receptor-β) 雌激素受體抗體
Anti-ESA (Epithelial Specific Antigen) 上皮特異性抗原抗體
Anti-ET-1 (Endothelin-1) 內(nèi)皮素-1抗體
Anti-ET-1(Endothelin-1) 內(nèi)皮素-1抗體
anti-ets1/E26 (E-twenty six 1) Ets轉(zhuǎn)錄因子家族ets1抗體
Anti-Eukaryotic aspartyl protease(Rice) 天冬氨酸蛋白酶家族蛋白抗體(水稻蛋白的一種)
Anti-EV71(Human enterovirus 71) 腸道病毒71型/手足口病病毒抗體
Anti-EXPB-2(beta-expansin)death inducer-obliterator-2 植物延展素-β
Anti-Fabp2 (fatty acid binding protein 2, intestinal) 脂肪酸結(jié)合蛋白2抗體
Anti-FABP(brain) (Fatty acid-binding protein brain) 腦型脂肪酸結(jié)合蛋白抗體
Anti-factor VIII(FVIII) 第八因子相關(guān)抗原抗體
Anti-factor VIII(FVIII) 第八因子相關(guān)抗原抗體
Anti-FADD (Fas-associated protein with death domain) Fas死亡結(jié)構(gòu)域相關(guān)蛋白
Anti-FAM3B/PANDER(PANcreatic DERived factor) 胰腺衍生因子抗體
Anti-FAS/Apo-a1/CD95(TNFR superfamily member;FasL receptor)(mouse rat) 載脂蛋白A1抗體
Anti-Apo-a1/apoA1 (Apolipoprotein A-1; APOA1 protein )human 載脂蛋白A1抗體
Anti-FasLAnti-FasL FasL抗體
Anti-FBN1 (fibrillin 1) 原纖維蛋白1抗體
Anti-Fe65 protein Fe65 蛋白抗體
Anti-FGF1/aFGF/ECGF-beta/HBGF-1 肝素結(jié)合生長因子/酸性成纖維細(xì)胞生長因子抗體
Anti-FGF3/Oncogene INT2(Fibroblast Growth Factor3) 纖維母細(xì)胞生長因子3抗體
Anti-FGF3/HSTF1/HBGF-4(Fibroblast Growth Factor4) 纖維母細(xì)胞生長因子4抗體
Anti-FGF5(Fibroblast Growth Factor 4) 纖維母細(xì)胞生長因子5抗體
Anti- KGF/FGF-7(Keratinocyte growth factor precursor) 角質(zhì)形成細(xì)胞生長因子受體/纖維母細(xì)胞生長因子-7抗體
Anti-FGF-8/HBGF-8 (Fibroblast growth factor 8) 成纖維細(xì)胞生長因子-8/纖維母細(xì)胞生長因子-8抗體
anti-FGF-10(Fibroblast Growth Factor-10) 成纖維細(xì)胞生長因子-10/纖維母細(xì)胞生長因子-10抗體
Anti-KGFR(kilodalton growth factor receptors) 角質(zhì)形成細(xì)胞生長因子受體抗體
Anti-FGFR1(Fibroblast Growth Factor Receptor 1) 纖維母細(xì)胞生長因子受體1抗體
Anti-FGFR2(Fibroblast Growth Factor Receptor 2) 成纖維細(xì)胞生長因子受體2抗體
Anti-FGFR3(Fibroblast Growth Factor Receptor 3) 成纖維細(xì)胞生長因子受體3抗體
Anti-FGFR4(Fibroblast Growth Factor Receptor 4) 成纖維細(xì)胞生長因子受體4抗體
Anti-fgl2 (Fibrinogen-like 2) 凝血酶原酶抗體/纖維介素蛋白/前凝血素抗體
anti-Fibulin 1 抗“衰老關(guān)鍵蛋白”抗體
Anti-Fibulin-5 抗“衰老關(guān)鍵蛋白”抗體
Anti-FLIP S/L (Flice-like Inhibitory Protein) 凋亡調(diào)節(jié)基因之一抗體
Anti-FLIPS (Flice-like Inhibitory Protein S) 凋亡調(diào)節(jié)基因之一抗體(短型)
Anti-FN (Fibronectin) 纖維連結(jié)蛋白抗體
Vacuum chamber (aluminum), QXT 9020940
Slideout electrical tray, SMC, QXT 9020913
Screw, centre rail clamp, CX1 9020681
Door, consumables, left, Cabinet Qsym 9020627
Belt, timing, GSC 9020483
Sheet, therm conductive, 30 x 30, GSC 9020480
PCB, signal distr., chassis AS, Qsym 9020393
Worktable, body parts, AS, Qsym 9020367
Tip slide, AS, Qsym 9020366
Electronic box, AS, Qsym 9020362
PCB - Sensor w/thermal fuse, RGQII 9020334
Adaptor, rotor disc 100, easylift, Cx2 9020287
Switch, assembly, lid, May03-Sep06, C1x 9020263
Plate for accessories, plete 9011906
Power Supply, 1000W, BR8 RD v1.0 9236991
Fan - rear 60x60, PyroQ96MD 9019145
Cog belt kit - X/Y, PyroQ96ID 9019119
PCB - Controller, PyroQ96ID 9019112
PyroMark Assay Design SW 2.0 9019077
PyroMark Storage Box 13x8x5cm, Pyro 9019074
Motor Z, EZ1xl 9019013
Motor P, EZ1xl 9019011
Tubing Set, Waste Wash,BR8000 9016060
X-Drive, Complete, BR8 RD AT3/B10 V20 9016056
Probe, 0.4 mm, BR8000 v1.01 9015725
Board, CPU 9015525
Interlock switch, for maintenance 9015521
Fan - peltier 60x60, PyroQ96MD 9019169
IPC mainboard, PyroQ96MD 9019155
PCB - Camera, PyroQ96MD 9019154
Tip-Tray Holder, 1100 μl tips 9014003
Labware Tracking Kit, BR8000 9013845
Controller, Gripper, VRA, DCU 9021176
PLC Module, CC-Link, DCU 9021081
Tubing connectors, vac.pump v2.0, BR8000 9020929
Needles 0.4x42mm (100), CX1 9020798
PCB, LED breakout, CAS 9020706
Clamp, table, single sided, C42 9020700
Panel, right, Cabinet Qsym 9020635
Packaging (plete), C12 9020325
Carrier, tubes, sample track, Qsym 9017659
Upgrade Kit Varispan, TA align., BR UNIV 9017324