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炔諾酮檢測試劑盒齊一生物科技(上海)有限公司專業(yè)經(jīng)營ELISA試劑盒產(chǎn)品種類齊全,質(zhì)量可靠,*,靈敏度高,效果穩(wěn)定,易保存,操作簡單.到貨快,確保每一個ELISA試劑盒質(zhì)量.凡是購買齊一生物科技有限公司ELISA試劑盒免費代測,提供技術(shù)服務(wù),5個工作日出實驗結(jié)果.歡迎新老客戶前來:
炔諾酮檢測試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1:1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的生物素標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測定結(jié)果.
在450nm波長處測定各孔的OD值.
Total CHOP Cell-Based Fluorometric ELISA Kit KA3269F
Total CBP 35 Cell-Based Fluorometric ELISA Kit KA3270F
Total GANP Cell-Based Fluorometric ELISA Kit KA3271F
Total Gastrin Cell-Based Fluorometric ELISA Kit KA3272F
Total GCG Cell-Based Fluorometric ELISA Kit KA3273F
Total mGluR-4 Cell-Based Fluorometric ELISA Kit KA3274F
Total GluR-5 Cell-Based Fluorometric ELISA Kit KA3275F
Total mGluR-6 Cell-Based Fluorometric ELISA Kit KA3276F
Total mGluR-7 Cell-Based Fluorometric ELISA Kit KA3277F
Total mGluR-8 Cell-Based Fluorometric ELISA Kit KA3278F
Total Glut1 Cell-Based Fluorometric ELISA Kit KA3279F
Total Glut3 Cell-Based Fluorometric ELISA Kit KA3280F
Total Granzyme B/H Cell-Based Fluorometric ELISA Kit KA3281F
Total HSP A9 Cell-Based Fluorometric ELISA Kit KA3282F
Total HSP A5 Cell-Based Fluorometric ELISA Kit KA3283F
Total Endoplasmin Cell-Based Fluorometric ELISA Kit KA3284F
Total GCS-β-1 Cell-Based Fluorometric ELISA Kit KA3285F
Total Choriogonadotropin β Cell-Based Fluorometric ELISA Kit KA3286F
Total Histone deacetylase 1 Cell-Based Fluorometric ELISA Kit KA3287F
Total Histone deacetylase 10 Cell-Based Fluorometric ELISA Kit KA3288F
Total SMAP45 Cell-Based Fluorometric ELISA Kit KA3289F
Total Histone deacetylase 6 Cell-Based Fluorometric ELISA Kit KA3290F
Total Histone deacetylase 7a Cell-Based Fluorometric ELISA Kit KA3291F
Total Histone deacetylase 9 Cell-Based Fluorometric ELISA Kit KA3292F
Total Neuregulin-1 SMDF Cell-Based Fluorometric ELISA Kit KA3293F
Total HSP10 Cell-Based Fluorometric ELISA Kit KA3294F
Total HSP105 Cell-Based Fluorometric ELISA Kit KA3295F
Total HSP40 Cell-Based Fluorometric ELISA Kit KA3296F
Total HSP60 Cell-Based Fluorometric ELISA Kit KA3297F
Total HSP90A Cell-Based Fluorometric ELISA Kit KA3298F
Total CSN3 Cell-Based Fluorometric ELISA Kit KA3299F
Total PRAF2 Cell-Based Fluorometric ELISA Kit KA3300F
Total Cytokeratin 14/16 Cell-Based Fluorometric ELISA Kit KA3301F
Total Cytokeratin 15 Cell-Based Fluorometric ELISA Kit KA3302F
Total Cytokeratin 16 Cell-Based Fluorometric ELISA Kit KA3303F
Total Cytokeratin 17 Cell-Based Fluorometric ELISA Kit KA3304F
Total Cytokeratin 18 Cell-Based Fluorometric ELISA Kit KA3305F
Total Cytokeratin 19 Cell-Based Fluorometric ELISA Kit KA3306F
Total Cytokeratin 20 Cell-Based Fluorometric ELISA Kit KA3307F
Total Cytokeratin 5 Cell-Based Fluorometric ELISA Kit KA3308F
Total Cytokeratin 7 Cell-Based Fluorometric ELISA Kit KA3309F
Total Cytokeratin 10 Cell-Based Fluorometric ELISA Kit KA3310F
Total Ki-67 Cell-Based Fluorometric ELISA Kit KA3311F
Total NOS2 Cell-Based Fluorometric ELISA Kit KA3312F
Total Ku-80 Cell-Based Fluorometric ELISA Kit KA3313F
Total Ku-70 Cell-Based Fluorometric ELISA Kit KA3314F
Total MAGE-1 Cell-Based Fluorometric ELISA Kit KA3315F
Total Mammaglobin A Cell-Based Fluorometric ELISA Kit KA3316F
Total Melan-A Cell-Based Fluorometric ELISA Kit KA3317F
Total Mcl-1 Cell-Based Fluorometric ELISA Kit KA3318F
Total BM28 Cell-Based Fluorometric ELISA Kit KA3319F
Total CDC46 Cell-Based Fluorometric ELISA Kit KA3320F
Total Mdm2 Cell-Based Fluorometric ELISA Kit KA3321F
Total MMP-1 Cell-Based Fluorometric ELISA Kit KA3322F
Total MMP-10 Cell-Based Fluorometric ELISA Kit KA3323F
Total MMP-11 Cell-Based Fluorometric ELISA Kit KA3324F
Total MMP-13 Cell-Based Fluorometric ELISA Kit KA3325F
Total MMP-14 Cell-Based Fluorometric ELISA Kit KA3326F
人腦膠質(zhì)細胞株(正常) HEB
人腦瘤細胞 SF126
人腦瘤細胞 SF763
人腦瘤細胞 SF767
人腦瘤細胞 SF17
人腦膠質(zhì)母細胞瘤細胞 TG-905
人膠質(zhì)瘤細胞 TJ905
人神經(jīng)母細胞瘤細胞 IMR-32
人神經(jīng)膠質(zhì)瘤細胞 U251
神經(jīng)膠質(zhì)瘤細胞 CRT
人膠質(zhì)母細胞瘤細胞 A172
神經(jīng)膠質(zhì)瘤細胞 SHG-44
人XG惡性膠質(zhì)瘤細胞 SF-295
人惡性膠質(zhì)母細胞瘤細胞 U87MG
人神經(jīng)上皮瘤細胞 SK-N-MC
人神經(jīng)上皮瘤細胞 SK-N-SH
人神經(jīng)上皮瘤細胞 SH-SY5Y
人腦神經(jīng)膠質(zhì)瘤細胞(H4) H4
人腦膠質(zhì)瘤細胞系 U373
人腦膠質(zhì)母細胞瘤細胞 SW038-C2
轉(zhuǎn)染了tk基因的SW038-C2細胞 SW038-C2-tk
人神經(jīng)膠質(zhì)瘤細胞 BT-325
人神經(jīng)母細胞瘤細胞 BE(2)C
人神經(jīng)母細胞瘤細胞 BE(2)-M17
人XG惡性膠質(zhì)瘤細胞 SF-295
人少突膠質(zhì)前體細胞-懸浮生長 HOPC-os
人跟骨髓瘤細胞 Hp2/o
人骨肉瘤細胞 U-2 OS
人骨肉瘤細胞 SP20
人骨肉瘤細胞 SW1353
人成骨肉瘤細胞 Saos-2
人骨肉瘤細胞 HOS
人骨髓瘤細胞 U266
人骨髓瘤細胞 3T3D
人成骨肉瘤細胞 MG-63
人多發(fā)性骨髓瘤細胞 RPMI-8226
人橫紋肌肉瘤細胞 A-204
胎兒骨髓間充質(zhì)細胞 FBM
人黑色素瘤細胞 HME1
人黑色素瘤細胞 HME4
人黑色素瘤細胞 HME6
人黑色素瘤細胞 HME7
人黑色素瘤細胞 HME2
人黑色素瘤細胞 HS-6
人黑色素瘤細胞 MV3
人黑色素瘤細胞 HSC1
人黑色素瘤細胞 NW38
人黑色素瘤細胞 B16
人黑色素瘤細胞 SK23
人黑色素瘤細胞 SK37
人黑色素瘤細胞 A875
人皮膚黑色素瘤細胞 SK-MEL-1
人惡性黑色素瘤細胞 A375
人侵襲性脈絡(luò)膜黑色素瘤細胞 M619
人黑色素瘤細胞 M14
人葡萄膜黑色素細胞 UM
人視網(wǎng)膜色素上皮細胞 D407
人視網(wǎng)膜母細胞瘤 Rb
人眼脈絡(luò)黑色素瘤細胞 OCM-1
人眼脈絡(luò)黑色素瘤細胞 MuM-2C
人眼脈絡(luò)黑色素瘤細胞 MuM-2B
人低侵襲性脈絡(luò)膜黑色素素瘤細胞 OCM-1A
人視網(wǎng)膜母細胞瘤 Y79
熒光素酶轉(zhuǎn)染人成骨肉瘤細胞 U2OS-Luc teT-on
人橫紋肌肉瘤細胞 TE671 subline No.2
人眼脈絡(luò)黑色素瘤細胞 C918
人橫紋癌細胞 A673
人惡性胚胎橫紋肌瘤細胞 RD
人表皮癌細胞 A-431
人正常皮膚細胞 HaCaT
人皮膚纖維微細胞系 Malmc
人皮膚成纖維細胞系 BJ
人成纖維肉瘤細胞 HT1080
人皮膚癌細胞系 HS-1
人皮膚癌細胞系 HS-4
人胸膜瘤細胞 SMC-1
人胸腺激酶缺陷性細胞系 143TK
人水痘帶狀皰疹病毒IgG(VZV-IgG)ELISA試劑盒
人乙酰膽堿酯酶(AChE)ELISA試劑盒
人B淋巴細胞刺激因子(Blys)受體ELISA試劑盒
人腸脂肪酸結(jié)合蛋白(iFABP)ELISA試劑盒
人糖化白蛋白(GA)ELISA試劑盒
人前列腺特異性抗原(PSA)ELISA試劑盒
人組織因子途徑抑制物(TFPI)ELISA試劑盒
人細胞間粘附分子1(ICAM-1/CD54)ELISA試劑盒
人血管內(nèi)皮細胞生長因子(VEGF)ELISA試劑盒
人抵抗素(Resistin)ELISA試劑盒
人胰高血糖素樣肽1(GLP-1)ELISA試劑盒
人載脂蛋白E(Apo-E)ELISA試劑盒
人單核細胞趨化蛋白1(MCP-1/CCL2/MCAF)ELISA試劑盒
人腦紅蛋白(NGB)ELISA試劑盒
人堿性成纖維細胞生長因子(bFGF)ELISA試劑盒
人脂氧素A4(LXA4)ELISA試劑盒
人脂聯(lián)素(ADP)ELISA試劑盒
人葡萄糖轉(zhuǎn)運蛋白1(GLUT1)ELISA試劑盒
人不對稱二甲基精氨酸(ADMA)ELISA試劑盒
人堿性成纖維細胞生長因子受體(bFGFR)ELISA試劑盒
人脂肪型脂肪酸結(jié)合蛋白(FABP4)ELISA試劑盒
人5,10亞甲基四氫葉酸還原酶(MTHFR)ELISA試劑盒
人組織相容性復(fù)合體Ⅰ類相關(guān)基因A(MICA)ELISA試劑盒
人細胞外信號調(diào)節(jié)激酶(ERK)ELISA試劑盒
人組織相容性復(fù)合體Ⅰ類相關(guān)基因B(MICB)ELISA試劑盒
人組織相容性復(fù)合體Ⅰ類相關(guān)基因D(MICD)ELISA試劑盒
人天門冬氨酸氨基轉(zhuǎn)移酶(AST)ELISA試劑盒
人抗肌聯(lián)蛋白抗體(TTN)ELISA試劑盒
人胸腺基質(zhì)淋巴生成素(TSLP)ELISA試劑盒
人糖連抗原153(CA153)ELISA試劑盒
人脂多糖(LPS)ELISA試劑盒
人膠質(zhì)纖維酸性蛋白(GFAP)ELISA試劑盒
人組織多肽抗原(TPA)ELISA試劑盒
人血管內(nèi)皮細胞粘附分子1(VCAM-1/CD106)ELISA試劑盒