5-Fluorouracil試劑簡介
5-Fluorouracil (5-FU) 是一種uracil的類似物 (nucleoside antimetabolite/analog),是一種有效的抗腫瘤藥。5-Fluorouracil 通過抑制胸苷酸合成酶影響嘧啶的合成,從而耗盡細(xì)胞內(nèi)dTTP 池。5-Fluorouracil 誘導(dǎo)細(xì)胞凋亡 (apoptosis),可用作化學(xué)敏化劑。5-Fluorouracil 還可抑制 HIV 病毒。5-Fluorouracil (5-FU) 可破壞外泌體特異性的 rRNA。
5-Fluorouracil試劑生物活性 | 5-Fluorouracil (5-FU) is an analogue of uracil and a potent antitumor agent. 5-Fluorouracil affects pyrimidine synthesis by inhibiting thymidylate synthetase thus depleting intracellular dTTP pools. 5-Fluorouracil induces apoptosis and can be used as a chemical sensitizer[1][2]. 5-Fluorouracil also inhibits HIV[3]. | ||||||||||||||||
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IC50 & Target[1][6] |
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體外研究 (In Vitro) | 5-Fluorouracil (5-Fu) and NSC 123127 (Dox) show synergistic anticancer efficacy. The IC50 value of 5-Fu/Dox-DNM toward human breast cancer (MDA-MB-231) cells is 0.25 μg/mL, presenting an 11.2-fold and 6.1-fold increase in cytotoxicity compared to Dox-DNM and 5-Fu-DNM, respectively[1]. In 5-fluorouracil (5-FU) and CDDP treated NFBD1-inhibited NPC cells, the NFBD1 expression in NPC CNE1 cell lines is depleted using lentivirus-mediated short hairpin RNA, and the sensitivity of these cells is elevated. NFBD1 knockdown leads to an obvious induction of apoptosis in CDDP- or 5-FU-treated CNE1 cells[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. | ||||||||||||||||
體內(nèi)研究 (In Vivo) | 5-Fluorouracil (23 mg/kg, 3 times/week) for 14 days, induces accelerated gastrointestinal transit associated with acute intestinal inflammation at day 3 after the start of treatment, which may have led to persistent changes in the ENS observed after days 7 and 14 of treatment contributing to delayed gastrointestinal transit and colonic dysmotility[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. | ||||||||||||||||
分子量 | 130.08 | ||||||||||||||||
性狀 | Solid | ||||||||||||||||
Formula | C4H3FN2O2 | ||||||||||||||||
CAS 號 | 51-21-8 | ||||||||||||||||
中文名稱 | 5-氟脲嘧啶;雙喃呋啶 | ||||||||||||||||
運(yùn)輸條件 | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
儲存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) | ||||||||||||||||
溶解性數(shù)據(jù) | In Vitro: DMSO : 50 mg/mL (384.38 mM; Need ultrasonic) 配制儲備液
* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。 In Vivo: 請根據(jù)您的實驗動物和給藥方式選擇適當(dāng)?shù)娜芙夥桨?。以下溶解方案都請先按?In Vitro 方式配制澄清的儲備液,再依次添加助溶劑: ——為保證實驗結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;體內(nèi)實驗的工作液,建議您現(xiàn)用現(xiàn)配,當(dāng)天使用; 以下溶劑前顯示的百
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