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上海啟達(dá)生物科技有限公司
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上海啟達(dá)生物科技有限公司
產(chǎn)品型號(hào)1*10^6cells/vial
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更新時(shí)間：2020-09-29 10:55:24瀏覽次數(shù)：706次
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人視網(wǎng)膜周細(xì)胞（HRPC）
背景介紹/context：
Human Retinal Pericyte Cells，These antibody-free human primary cells were initiated by centrifugal elutriation following dispersal of normal retinal tissue with collagenase and dispase without the use of positive selection of antibodies. 人視網(wǎng)膜周細(xì)胞提取于正常視網(wǎng)膜組織，經(jīng)酶消化純化，不使用任何陽性抗體篩選，細(xì)胞無抗性。
動(dòng)物種別/Organism：人
組織來源 Tissue and Cell Type：視網(wǎng)膜組織
形態(tài)/Morphology：上皮細(xì)胞樣，貼壁生長
培養(yǎng)基和添加劑/Complete Growth Medium and Culture Conditions：
*培養(yǎng)基：CultureBoost™經(jīng)典細(xì)胞培養(yǎng)基（貨號(hào)：4N0-500）
氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。
*凍存液：細(xì)胞凍存液Cell Freezing Medium（4Z0-705）
人視網(wǎng)膜周細(xì)胞（HRPC)出庫檢測指標(biāo)：
| Standard Tests | |
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
| Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) | Negative |
| Miscellaneous Tests | |
| TESTS | RESULTS |
| Cytoplasmic alpha-Actinin Filaments | > 80% positive by immunofluorescence |
| Cytoplasmic Desmin Intermediate Filaments | > 80% positive by immunofluorescence |
| Cytoplasmic VWF / Factor VIII | < 0.2% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | < 0.2% positive by immunofluorescence |
| NG2 (Neural/Glial Antigen 2) | >90% positive by immunofluorescence |
| PDGFR-beta (Plaet Derived Growth Factor Receptor beta) | >90% positive by immunofluorescence |
| GFAP (Glial Fibrillary Acidic Protein) | < 1% by immunofluorescence |
| Glutamine Synthetase | < 1% by immunofluorescence |
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